Composite

Part:BBa_K4501019

Designed by: Pau Marín Escudero   Group: iGEM22_Barcelona_UB   (2022-09-22)


myc shRNA C/D-box

This part contains the expression cassette for our shRNA against myc, followed by a GFP expression unit that allows an easy reporter of the transfection and expression. The shRNA is labelled on the 3' terminus with the C/D-box, which allows the loading of the shRNA into the exosomes via L7Ae.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1769


[edit]
Categories
Parameters
None